Zika Virus
Khoom npe
HWTS-FE002 Zika Virus Nucleic Acid Detection Kit (Fluorescence PCR)
Daim ntawv pov thawj
CE
Kab mob sib kis
Tus kab mob Zika belongs rau genus Flaviviridae, yog ib leeg-stranded zoo-stranded RNA tus kab mob nrog ib txoj kab uas hla ntawm 40-70nm.Nws muaj ib lub hnab ntawv, muaj 10794 nucleotides, thiab encodes 3419 amino acids.Raws li genotype, nws tau muab faib ua African hom thiab Asian hom.Tus kab mob Zika yog tus kab mob kis tus kheej los ntawm tus kab mob Zika, uas feem ntau kis los ntawm cov yoov tshaj cum Aedes aegypti.Cov yam ntxwv kho mob feem ntau yog kub taub hau, ua pob liab vog, mob caj dab los yog conjunctivitis, thiab nws tsis tshua muaj neeg tuag.Raws li World Health Organization, neonatal microcephaly thiab Guillain-Barre syndrome (Guillain-Barré syndrome) tuaj yeem cuam tshuam nrog tus kab mob Zika.
Channel
| FAM | Zika kab mob nucleic acid |
| ROX | Internal Control |
Technical Parameters
| Cia | ≤30 ℃ & tiv thaiv los ntawm lub teeb |
| Txee lub neej | 12 hli |
| Hom Qauv | tshiab serum |
| Ct | ≤38 |
| CV | ≤ 5.0% |
| LoD | 500 ng / μL |
| Qhov tshwj xeeb | Cov txiaj ntsig tau txais los ntawm cov khoom siv no yuav tsis cuam tshuam los ntawm hemoglobin (<800g / L), bilirubin (<700μmol / L), thiab ntshav lipids / triglycerides (<7mmol / L) hauv cov ntshav. |
| Cov cuab yeej siv tau | ABI 7500 Real-Time PCR Systems ABI 7500 Fast Real-Time PCR Systems QuantStudio®5 Real-Time PCR Systems SLAN-96P Real-Time PCR Systems LightCycler®480 Real-Time PCR system LineGene 9600 Plus Real-Time PCR Detection System MA-6000 Real-Time Quantitative Thermal Cycler BioRad CFX96 Real-Time PCR System, BioRad CFX Opus 96 Real-Time PCR System |
Ua hauj lwm Flow
Kev xaiv 1.
QIAamp Viral RNA Mini Kit (52904), Nucleic Acid Extraction lossis Purification Reagent (YDP315-R) los ntawm Tiangen Biotech (Beijing) Co., Ltd.Kev rho tawmyuav tsum tau muab rho tawm raws li cov lus qhia extraction, thiab qhov pom zoo extraction ntim yog 140 μL thiab qhov pom zoo elution ntim yog 60 μL.
Kev xaiv 2.
Macro & Micro-Test Viral DNA/RNA Kit (HWTS-3004-32, HWTS-3004-48, HWTS-3004-96) thiab Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006).Kev rho tawm yuav tsum tau muab rho tawm raws li cov lus qhia.Cov qauv rho tawm yog 200 μL, thiab qhov pom zoo elution ntim yog 80μL.
