15 Hom Kev pheej hmoo siab Human Papillomavirus E6/E7 Gene mRNA
Khoom npe
HWTS-CC005A-15 Hom kev pheej hmoo siab Human Papillomavirus E6/E7 Gene mRNA Detection Kit (Fluorescence PCR)
Kab mob sib kis
Kev mob khees xaws ncauj tsev menyuam yog ib yam ntawm cov poj niam mob qog noj ntshav thoob plaws ntiaj teb, thiab nws qhov tshwm sim muaj feem xyuam nrog tib neeg papillomaviruses (HPV), tab sis tsuas yog ib feem me me ntawm cov kab mob HPV tuaj yeem ua rau mob qog noj ntshav.High-risk HPV kis kab mob ntawm ncauj tsev menyuam epithelial hlwb thiab tsim ob oncoproteins, E6 thiab E7.Cov protein no tuaj yeem cuam tshuam rau ntau yam cellular proteins (xws li qog nqaij hlav proteins pRB thiab p53), ua kom lub voj voog ntawm tes, cuam tshuam rau DNA synthesis thiab genome stability, thiab cuam tshuam nrog cov tshuaj tiv thaiv kab mob thiab tshuaj tiv thaiv kab mob.
Channel
| Channel | Cheebtsam | Genotype kuaj |
| FAM | HPV Reaction Buffer 1 | HPV 16, 31, 33, 35, 51, 52, 58 |
| VIC/HEX | Tib neeg beta-actin gene | |
| FAM | HPV Reaction Buffer 2 | HPV 18, 39, 45, 53, 56, 59, 66, 68 |
| VIC/HEX | Tib neeg INS gene |
Technical Parameters
| Cia | Ua kua: ≤-18 ℃ |
| Txee lub neej | 9 muaj |
| Hom Qauv | cervical exfoliated cell |
| Ct | ≤38 |
| CV | ≤ 5.0% |
| LoD | 500 daim / ml |
| Cov cuab yeej siv tau | Siv Biosystems 7500 Real-Time PCR System Siv Biosystems 7500 Fast Real-Time PCR Systems QuantStudio®5 Real-Time PCR Systems SLAN-96P Real-Time PCR Systems LightCycler® 480 Real-Time PCR system LineGene 9600 Plus Real-Time PCR Detection System MA-6000 Real-Time Quantitative Thermal Cycler BioRad CFX96 Real-Time PCR System BioRad CFX Opus 96 Real-Time PCR System |
Ua hauj lwm Flow
Pom zoo extraction reagent: Macro & Micro-Test Viral DNA/RNA Kit (HWTS-3020-50-HPV15) los ntawm Jiangsu Macro & Micro-Test Med-Tech Co., Ltd. Cov extraction yuav tsum tau ua nruj me ntsis raws li cov lus qhia siv .Qhov pom zoo elution ntim yog 50μL.Yog tias tus qauv tsis zom tag nrho, rov qab mus rau qib 4 rau kev rov ua dua tshiab.Thiab tom qab ntawd sim raws li cov lus qhia rau kev siv.
Pom zoo extraction reagent: RNAprep Ntshiab Tsiaj Tissue Total RNA Extraction Kit (DP431).Kev rho tawm yuav tsum tau ua raws li cov lus qhia rau kev siv nruj me ntsis (Nyob rau hauv kauj ruam 5, muab ob npaug rau cov concentration ntawm DNaseI ua hauj lwm tov, uas yog, noj 20μL ntawm RNase-Free DNaseI (1500U) Tshuag tov rau hauv ib tug tshiab RNase-Free centrifuge raj, ntxiv 60μL ntawm RDD tsis, thiab sib tov maj mam).Qhov pom zoo elution ntim yog 60μL.Yog tias tus qauv tsis tau zom tag nrho, rov qab mus rau qib 5 rau kev rov ua dua tshiab.Thiab tom qab ntawd sim raws li cov lus qhia rau kev siv.
