Mycobacterium Tuberculosis Isoniazid Resistance Mutation

Lus piav qhia luv luv:

Cov khoom siv no tsim nyog rau kev tshawb nrhiav qhov zoo ntawm qhov chaw hloov pauv tseem ceeb hauv tib neeg cov hnoos qeev cov qauv sau los ntawm Tubercle bacillus zoo cov neeg mob uas ua rau mycobacterium tuberculosis isoniazid tsis kam: InhA txhawb cheeb tsam -15C>T, -8T>A, -8T>C;AhpC txhawb nqa cheeb tsam -12C>T, -6G>A;Homozygous mutation ntawm KatG 315 codon 315G> A, 315G> C .

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Product Detail

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HWTS-RT137 Mycobacterium Tuberculosis Isoniazid Resistance Mutation Detection Kit (Melting Curve)

Kab mob sib kis

Mycobacterium tuberculosis, luv luv li Tubercle bacillus (TB), yog cov kab mob pathogenic uas ua rau tuberculosis.Tam sim no, cov tshuaj tiv thaiv kab mob ua ntej siv feem ntau suav nrog isoniazid, rifampicin thiab hexambutol, thiab lwm yam. Cov tshuaj tiv thaiv kab mob thib ob muaj xws li fluoroquinolones, amikacin thiab kanamycin, thiab lwm yam. Cov tshuaj tsim tshiab yog linezolid, bedaquiline thiab delamani, thiab lwm yam. .Txawm li cas los xij, vim yog siv cov tshuaj tiv thaiv kab mob tsis raug thiab cov yam ntxwv ntawm lub cell phab ntsa qauv ntawm mycobacterium tuberculosis, mycobacterium tuberculosis tsim cov tshuaj tiv thaiv rau cov tshuaj tiv thaiv tuberculosis, uas ua rau muaj teeb meem loj rau kev tiv thaiv thiab kho tuberculosis.

Channel

FAM	MP nucleic acid
ROX	Internal Control

Technical Parameters

Cia	≤-18 ℃
Txee lub neej	12 hli
Hom Qauv	hnoos qeev
CV	≤ 5.0%
LoD	Qhov kev tshawb pom txwv rau cov kab mob qus-hom isoniazid resistant yog 2x103 kab mob / mL, thiab qhov kev kuaj pom cov kab mob mutant yog 2x103 kab mob / mL.
Qhov tshwj xeeb	ib.Tsis muaj cov tshuaj tiv thaiv ntawm tib neeg genome, lwm yam nontuberculous mycobacteria thiab kab mob ntsws uas kuaj pom los ntawm cov khoom siv no. b.Cov chaw hloov pauv ntawm lwm cov tshuaj tiv thaiv kab mob hauv cov tsiaj qus Mycobacterium tuberculosis, xws li kev tiv thaiv kev txiav txim siab thaj tsam ntawm rifampicin rpoB noob, tau kuaj pom, thiab cov txiaj ntsig kev sim pom tias tsis muaj kev tiv thaiv rau isoniazid, qhia tias tsis muaj qhov cuam tshuam.
Cov cuab yeej siv tau	SLAN-96P Real-Time PCR Systems BioRad CFX96 Real-Time PCR Systems LightCycler480® Real-Time PCR System

Ua hauj lwm Flow

Yog siv Macro & Micro-Test General DNA/RNA Kit (HWTS-3019) (uas tuaj yeem siv nrog Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006C, HWTS-3006B)) los ntawm Jiangsu Macro & Micro-Test Med-Tech Co., Ltd. rau kev rho tawm, ntxiv 200μL ntawm cov kev tswj tsis zoo thiab ua cov hnoos qeev kom raug kuaj ua ntu zus, thiab ntxiv 10μL ntawm kev tswj sab hauv cais rau hauv kev tswj tsis zoo, ua cov hnoos qeev los kuaj, thiab cov kauj ruam tom ntej yuav tsum tau ua nruj me ntsis raws li cov lus qhia extraction.Qhov ntim tau muab rho tawm yog 200μL, thiab qhov pom zoo elution ntim yog 100μL.