Human Papillomavirus (28 Hom) Genotyping Detection Kit (Fluorescence PCR)
Khoom npe
HWTS-CC004A-Human Papillomavirus (28 Hom) Genotyping Detection Kit (Fluorescence PCR)
Kab mob sib kis
Cov khoom siv siv ntau yam nucleic acid amplification (PCR) fluorescence detection method.Cov primers tshwj xeeb thiab cov tshuaj ntsuam xyuas yog tsim los ntawm L1 noob hom phiaj ntawm HPV.Kev sojntsuam tshwj xeeb yog sau nrog FAM fluorophore (HPV6, 16, 26, 40, 53, 58, 73), VIC/HEX fluorophore (HPV11, 18, 33, 43, 51, 59, 81), CY5 fluorophore (HPV35, 44) , 45, 54, 56, 68, 82) thiab ROX fluorophore (HPV31, 39, 42, 52, 61, 66, 83) ntawm 5', thiab 3' quencher pawg yog BHQ1 lossis BHQ2.Thaum lub sij hawm PCR amplification, tshwj xeeb primers thiab probes khi rau lawv cov hom phiaj sib lawv liag.Thaum Taq enzyme ntsib cov kev sojntsuam khi rau lub hom phiaj ib ntus, nws ua haujlwm ntawm 5 'kawg exonuclease kom cais cov neeg sau xov xwm fluorophore los ntawm quencher fluorophore, kom lub fluorescence xyuas qhov system tuaj yeem tau txais cov teeb liab fluorescent, uas yog, txhua zaus DNA. strand yog amplified, ib tug fluorescent molecule yog tsim, uas paub qhov ua tiav synchronization ntawm tsub zuj zuj ntawm fluorescent teeb liab thiab tsim cov khoom PCR, thiaj li mus cuag qualitative thiab genotyping nrhiav pom nucleic acids ntawm 28 hom tib neeg papillomavirus nyob rau hauv lub ncauj tsev menyuam exfoliated cell kuaj. .
Channel
| FAM | 16, 58, 53, 73, 6, 26, 40 · |
| VIC/HEX | 18, 33, 51, 59, 11, 81, 43 |
| ROX | 31, 66, 52, 39, 83, 61, 42 |
| CY 5 | 56, 35, 45, 68, 54, 44, 82 |
Technical Parameters
| Cia | ≤-18 ℃ Hauv qhov tsaus ntuj |
| Txee lub neej | 12 hli |
| Hom Qauv | Cervical exfoliated cell |
| Ct | ≤ 25 |
| CV | ≤ 5.0% |
| LoD | 25 daim ntawv / cov lus teb |
| Cov cuab yeej siv tau | Yooj Yim Amp Real-time Fluorescence Isothermal Detection System (HWTS1600)
Siv Biosystems 7500 Real-Time PCR Systems
Siv Biosystems 7500 Fast Real-Time PCR Systems
QuantStudio®5 Real-Time PCR Systems
SLAN-96P Real-Time PCR Systems
LightCycler® 480 Real-Time PCR system
LineGene 9600 Plus Real-Time PCR Detection System
MA-6000 Real-Time Quantitative Thermal Cycler
BioRad CFX96 Real-Time PCR System
BioRad CFX Opus 96 Real-Time PCR System |
Ua hauj lwm Flow
Kev xaiv 1.
Pom zoo extraction reagent: Macro & Micro-Test Sample Release Reagent (HWTS-3005-8).
Kev xaiv 2.
Pom zoo extraction reagent: Macro & Micro-Test Viral DNA/RNA Kit (HWTS-3004-32, HWTS-3004-48, HWTS-3004-96) thiab Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006).
