Tib neeg BRAF Gene V600E Mutation
Khoom npe
HWTS-TM007-Human BRAF Gene V600E Mutation Detection Kit (Fluorescence PCR)
Daim ntawv pov thawj
CE
Kab mob sib kis
Ntau tshaj 30 hom kev hloov pauv ntawm BRAF tau pom, ntawm 90% yog nyob rau hauv exon 15, qhov twg V600E kev hloov pauv tau suav tias yog kev hloov pauv ntau tshaj plaws, uas yog, thymine (T) ntawm txoj haujlwm 1799 hauv exon 15 yog hloov mus rau adenine (A), uas ua rau hloov valine (V) ntawm txoj hauj lwm 600 los ntawm glutamic acid (E) hauv cov khoom protein.BRAF kev hloov pauv feem ntau pom muaj nyob rau hauv cov qog nqaij hlav xws li melanoma, mob qog noj ntshav, mob qog noj ntshav, thiab mob ntsws cancer.Kev nkag siab txog kev hloov pauv ntawm BRAF noob tau dhau los ua qhov yuav tsum tau tshuaj xyuas EGFR-TKIs thiab BRAF noob hloov pauv cov tshuaj hauv kev kho mob tshuaj rau cov neeg mob uas tuaj yeem tau txais txiaj ntsig.
Channel
| FAM | V600E kev hloov pauv, kev tswj sab hauv |
Technical Parameters
| Cia | ≤-18 ℃ |
| Txee lub neej | 9 muaj |
| Hom Qauv | paraffin-embedded pathological cov ntaub so ntswg |
| CV | 5.0% |
| Ct | ≤38 |
| LoD | Siv cov khoom siv los kuaj xyuas qhov sib raug zoo ntawm LoD.a) nyob rau hauv 3ng / μL hom tsiaj qus tom qab, 1% kev hloov pauv tuaj yeem kuaj pom hauv cov tshuaj tiv thaiv tsis ruaj khov;b) nyob rau hauv 1% kev hloov pauv, qhov hloov pauv ntawm 1 × 103Cov ntawv luam / mL hauv hom tsiaj qus tom qab ntawm 1 × 105Cov ntawv luam / mL tuaj yeem kuaj tau ruaj khov hauv cov tshuaj tiv thaiv tsis zoo;c) IC Reaction Buffer tuaj yeem kuaj xyuas qhov qis tshaj qhov txwv tsis pub muaj kev tswj hwm SW3 ntawm lub tuam txhab kev tswj hwm sab hauv. |
| Cov cuab yeej siv tau: | Siv Biosystems 7500 Real-Time PCR SystemsSiv Biosystems 7300 Real-Time PCR Systems, QuantStudio® 5 Real-Time PCR Systems LightCycler® 480 Real-Time PCR system BioRad CFX96 Real-Time PCR System |
Ua hauj lwm Flow
Pom zoo extraction reagents: QIAGEN's QIAamp DNA FFPE Tissue Kit (56404), Paraffin-embedded Tissue DNA Rapid Extraction Kit (DP330) tsim los ntawm Tiangen Biotech (Beijing) Co., Ltd.
